Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait.
نویسندگان
چکیده
This work was supported by grants from the U.S. Public Health Service and the National Science Foundation. The generosity and interest of Francis J. Ryan is gratefully acknowledged. The work was greatly abetted by the enthusiastic assistance of Sydell Fleischer, Phyllis Fried, Charlotte Marfey, and Christine Thomas. 1 Correns, C., Ber. dtsch. bot. Ges., 36, 686 (1908). 2 Rhoades, M. M., in Encycl. Plant Physiol., 1, 19 (1955). 3 Caspari, E., Adv. Genetics, 2, 2 (1948). 4 Sager, R., these PROCEEDINGS, 40, 356 (1954). 6 Sager, R., Science, 132, 1459 (1960). 6 Sager, R., and Y. Tsubo, Zeitsch. Vererb., 92, 430 (1961). Cavalli-Sforza, L. L., and J. Lederberg, Sixth International Congress on Microbiology, Rome (Springfield, Illinois: Charles C Thomas, 1953), p. 108. 8 Luria, S. E., and M. Delbruck, Genetics, 28, 491 (1943). 9 Newcombe, H. B., Nature, 164, 150 (1949). 10 Sager, R., and Y. Tsubo, Arch. Mikrobiol., -42, 159 (1962). 11 Sager, R., and Y. Tsubo, in preparation. 12 Sager, R., Genetics, 40, 476 (1955). 13 Lederberg, J., and E. M. Lederberg, J. Bact. 63, 399 (1952). 14 Cavalli-Sforza, L. L., and J. Lederberg, Genetics, 41, 367 (1956). 16 Cavalli-Sforza, L. L., in Drug Resistance in Micro-organisms, ed. G. E. W. Wolstenholme and C. M. O'Connor (London: Churchill, Ltd., 1957). 16 Cohen, S. S., J. Biol. Chem., 168, 511 (1947).
منابع مشابه
Optimization of Agrobacterium-mediated transformation in oyster mushroom (Pleurotus ostreatus) by vector containing human pro-insulin gene
Transferring foreign genes into mushroom mediated by Agrobacterium tumefaciens is a standard technique in genetic engineering. Recombinant human insulin has been greatly used in the treatment of type I diabetes. The production of edible mushroom derived insulin should facilitate oral delivery. In this study we used the Agrobacterium tumefaciens mediated transformation method for the transfer an...
متن کاملIdentification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172
Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA br...
متن کاملAgrobacterium-mediated transformation of cotyledonary leaf of lettuce (Lactuca sativa L.) by the GCHI gene
Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
متن کاملshRNA-mediated downregulation of α-N-Acetylgalactosaminidase inhibits migration and invasion of cancer cell lines
Objective(s): Extracellular matrix (ECM) is composed of many kinds of glycoproteins containing glycosaminoglycans (GAGs) moiety. The research was conducted based on the N-Acetylgalactosamine (GalNAc) degradation of ECM components by α-N-acetylgalactosaminidase (Nagalase) which facilitates migration and invasion of cancer cells. This study aims to investigate the effects of Naga-shRNA downregula...
متن کاملAn intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA.
We report here the identification of a cell line containing single and double missense mutations in cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. When present in homoplasmy, the single mutant displays a normal complex IV assembly but a significantly reduced COX activity, while the double mutant almost completely compensates the functional defect of the first mutation. We...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 48 شماره
صفحات -
تاریخ انتشار 1962